rabbit anti il20rb antibody (Proteintech)
Structured Review

Rabbit Anti Il20rb Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti il20rb antibody/product/Proteintech
Average 91 stars, based on 3 article reviews
Images
1) Product Images from "IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer."
Article Title: IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer.
Journal: Journal of translational medicine
doi: 10.1186/s12967-023-04800-5
Figure Legend Snippet: Fig. 3 IL20RB promotes stemness and chemoresistance in pancreatic cancer in vivo. A–B Tumor formation results after subcutaneous injection of 105, 106 and 107 PANC-1, respectively, into nude mice. C Tumor growth curve (n = 5 per group). D Statistical analysis of tumor weight at the end of the trial (n = 5 per group). E–F Representative immunohistochemical (IHC) images of NANOG and SOX2 in tumor tissue sections and statistical analysis of IHC scores, scale bar = 40 µm. G Western blot analysis of NANOG, SOX2 and IL20RB in tumor tissues. (H) 10^7 PANC-1 was injected subcutaneously into nude mice, and then gemcitabine 100 mg/kg was intraperitoneally injected twice a week for 4 weeks. I Statistical analysis of tumor weight at the endpoint. J Tumor growth curve. K–L Representative IHC images of pan-CK in tumor tissue sections and statistical analysis of IHC scores, scale bar = 40 µm
Techniques Used: In Vivo, Injection, Immunohistochemical staining, Western Blot
Figure Legend Snippet: Fig. 4 IL20RB activates STAT3 phosphorylation to promote pancreatic cancer stemness and chemotherapy resistance. A Western blot analysis of IL20RB, STAT3 and p-STAT3 (Tyr705) proteins in pancreatic cancer cell line MIA PaCa-2. B Western blot analysis of IL20RB, STAT3 and p-STAT3 (Tyr705) in pancreatic cancer cell line PANC-1. C Real-time quantitative PCR analysis of the mRNA expression of IL20RB, NANOG, SOX2 and POU5F1 in MIA PaCa-2 treated with the vehicle or STAT3 inhibitor (Stattic), respectively (n = 3 in each group). (D) Western blot analysis of IL20RB, STAT3, p-STAT3 (Tyr705), NANOG and SOX2 proteins in MIA PaCa-2 after the vehicle or Stattic treatment. E Sphere-forming assay and statistical analysis of MIA PaCa-2 after treatment with the vehicle or Stattic (n = 3 per group). F Colony formation assay and statistical analysis of MIA PaCa-2 treated with 10 nM gemcitabine for 24 h with or without Stattic (n = 3 per group). G Representative immunofluorescence images of IL20RB, pan-CK and p-STAT3 in human pancreatic cancer tissue sections, scale bar = 40 µm
Techniques Used: Phospho-proteomics, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Colony Assay, Immunofluorescence